A vascularized bone-on-a-chip model development via exploring mechanical stimulation for evaluation of fracture healing therapeutics
نویسندگان
چکیده
Bone is the major connective tissue maintaining structural integrity of human body. However, fracture and many skeletal degenerative diseases can compromise this function. Thus, therapeutics related to bone degeneration are significant research interest require good in vitro models for such therapeutic evaluation. a highly vascularized incorporation feature significantly important mimicking osteogenic microenvironment. In current study, we developed flat model via simultaneous mechanical actuation strain fluid shear. The was achieved by static magnetic field nanocomposite scaffold. shear generated developing micropattern on replica molding laser-based microfabrication. From live cell imaging window microdevice, both vasculature like cellular morphology observed. SEM study showed thick ECM deposition dynamic culture. PCR (Col-1, osteocalcin) angiogenic phenotypes (PECAM) were observed culture scaffolds while chondrogenic marker (Col-2) downregulated.
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ژورنال
عنوان ژورنال: In vitro models
سال: 2021
ISSN: ['2731-3433', '2731-3441']
DOI: https://doi.org/10.1007/s44164-021-00004-7